Team:NJAU China/Parts

Label	Name	Type	Description	Designer	Length
	BBa_K1649000	Composite	red light sensor	Yang Yang	4000bp
	BBa_K1649001	Composite	red light sensor with a GFP reporter	Yang Yang	4883bp
Favorite	BBa_K1649002	Composite	Red light sensor with TetR not gate and GFP reporter	Yang Yang	7280bp
Favorite	BBa_K1649003	Composite	YF1 blue light sensor with GFP reporter	Yang Yang	3117bp
	BBa_K1649004	Composite	YF1-FixJ with terminator	Yang Yang	1933bp

BBa_K1649000

Red Light Sensor

T

he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.

BBa_K1649001

Red Light Sensor with a GFP Reporter

G

FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.

BBa_K1649002

Red Light Sensor with TetR Not Gate and GFP Reporter

A

s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.

BBa_K1649004

YF1-FixJ with Terminator

T

he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.

BBa_K1649003

YF1 Blue Light Sensor with GFP Reporter

G

FP is used to report the function of blue light sensor.